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Image Search Results
Journal: The Journal of investigative dermatology
Article Title: Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.
doi: 10.1046/j.1523-1747.2003.12319.x
Figure Lengend Snippet: Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Article Snippet: After the overnight incubation (room temperature) with primary
Techniques: Expressing, Incubation